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KMID : 1098420040120050415
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Korean Journal of Medicinal Crop Science
2004 Volume.12 No. 5 p.415 ~ p.423
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Myristicae Semen Extract Protects Excitotoxicity in Cultured Neuronal Cells
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Kim Ji-Ye
Ban Ju-Yeon
Bang Kyung-Hwan
Seong Nak-Sul
Song Kyung-Sik
Bae Ki-Hwan
Seong Yeon-Hee
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Abstract
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Myristica fragrans seed from Myristica fragrans Houtt (Myristicaceae) has various pharmacological activities peripherally and centrally. The present study aims to investigate the effect of the methanol extract of Myristica fragrans seed (MF) on kainic acid (KA)-induced neurotoxicity in primary cultured rat cerebellar granule neuron. MF, over a concentration range of 0.05 to 5;{mu}g/ml inhibited KA (500;{mu}M)-induced neuronal cell death, which was measured by trypan blue exclusion test and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. MF (0.5;{mu}g/ml) inhibited glutamate release into medium induced by KA (500;{mu}M), which was measured by HPLC. Pretreatment of MF (0.5;{mu}g/ml) inhibited KA (500;{mu}M)-induced elevation of cytosolic calcium concentration ([Ca^{2+}]_c), which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that MF prevents KA-induced neuronal cell damage in vitro.
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KEYWORD
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Myristica fragrans seed, kainic acid, neurotoxicity, cerebellar granule cells
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